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KMID : 0545120200300121912
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Journal of Microbiology and Biotechnology
2020 Volume.30 No. 12 p.1912 ~ p.1918
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Evaluation of 2,3-Butanediol Production from Red Seaweed Gelidium amansii Hydrolysates Using Engineered Saccharomyces cerevisiae
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Ra Chae-Hun
Seo Jin-Ho
Jeong Gwi-Taek
Kim Sung-Koo
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Abstract
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Hyper-thermal (HT) acid hydrolysis of red seaweed Gelidium amansii was performed using 12% (w/v) slurry and an acid mix concentration of 180 mM at 150°C for 10 min. Enzymatic saccharification when using a combination of Celluclast 1.5 L and CTec2 at a dose of 16 U/ml led to the production of 12.0 g/l of reducing sugar with an efficiency of enzymatic saccharification of 13.2%. After the enzymatic saccharification, 2,3-butanediol (2,3-BD) fermentation was carried out using an engineered S. cerevisiae strain. The use of HT acid-hydrolyzed medium with 1.9 g/l of 5-hydroxymethylfurfural showed a reduction in the lag time from 48 to 24 h. The 2,3-BD concentration and yield coefficient at 72 h were 14.8 g/l and 0.30, respectively. Therefore, HT acid hydrolysis and the use of the engineered S. cerevisiae strain can enhance the overall 2,3-BD yields from G. amansii seaweed.
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KEYWORD
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Hyper-thermal acid hydrolysis, enzymatic saccharification, Gelidium amansii, 2,3-butanediol, engineered Saccharomyces cerevisiae
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